How does EDTA work in the blood?

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EDTA tube is the standard tube used in clinical hematology, crossmatch, blood grouping and various blood cell analysis instruments. The additive EDTA acts as a powerful anticoagulant by binding to calcium in the blood.

How does EDTA work as an anticoagulant?

EDTA Foundation

It acts as a chelator (sequestrant) of Ca++, which plays a very important role in carrying out the coagulation process, thus preventing the formation of fiber.

What does EDTA do in the blood?

The anticoagulant EDTA, also known as ethylenediaminetetraacetic acid, is a chemical that binds to metal ions such as calcium, magnesium, lead, and iron. Because of this, it is used in medicine to prevent blood clots and to remove calcium and lead from the body.

What are the anticoagulants used in the tubes?

The most commonly used anticoagulants are: EDTA, sodium citrate, heparin, oxalates.

How does sodium citrate work as a blood thinner?

Citrate acts as an anticoagulant by being a chelator of calcium ions. Dextrose provides a substrate for glycolysis during storage, thus extending the life span of erythrocytes.

36 related questions found

How is sodium citrate anticoagulant prepared?


  • For every 5 ml of blood, add 1 ml of sodium citrate solution.
  • Its use has been restricted to coagulation studies.

How does citrate work?

Magnesium citrate is used to treat short-term constipation. Magnesium citrate is in a class of medications called saline laxatives. It works by causing water to be retained with the stool. This increases the number of bowel movements and softens the stool so that it is easier to pass.

What tests are performed in the red tube?

Red cap: There is no anticoagulant in this tube and it is used for biochemical tests (glucose, cholesterol, triglycerides), tumor markers, hormones, or antibodies (the AIDS virus).

What is a Vacutainer tube?

1. The Vacutainer or Sample Tube is a test tube specifically designed for venipuncture. … This is a vacuum PET plastic or glass tube with a soft plastic stopper, which allows a needle to pass through it with slight pressure.

What types of anticoagulants are the most used in hematology?


  • EDTA (ETHYLENE-DIAMINO-TETRA-ACETATE): It is the preferred anticoagulant for cell counts and morphological studies.
  • HEPARIN: It is used both in routine and specialized studies.

What is EDTA and what tests is it used for?

EDTA tube is the standard tube used in clinical hematology, crossmatch, blood grouping and various blood cell analysis instruments. The additive EDTA acts as a powerful anticoagulant by binding to calcium in the blood.

What is EDTA K2?

It is the ideal additive for carrying out the count of leukocytes, platelets, and red blood cells and also for the determination of the leukocyte formula, flow cytometry and lead determination, since the morphology of blood cells is preserved.

What is EDTA Pseudothrombocytopenia?

Introduction: Pseudothrombocytopenia induced by EDTA (ethylene diamine tetraacetic acid) is a platelet agglutination phenomenon that occurs in vitro, mediated by anti-platelet antibodies of the IgG, IgA or IgM type directed against the glycoprotein IIb/IIIa complex of the platelet membrane.

What do anticoagulants do?

Anticoagulants are medications that prevent the formation of blood clots. They also prevent existing blood clots from getting larger. Clots in the arteries, veins, and heart can cause heart attacks, strokes, and blockages.

What test is taken in the black tube?

Black and/or gray tube • Contains sodium fluoride. It is used to obtain plasma. Blood glucose determination tests. It allows the preservation of the sample for 1 hour without changing the result.

What do the colors on the Vacutainer tubes mean?

There are several types of Vacutainer that differ in the color of their cap. … Each stopper color indicates the additive, or lack thereof, contained in the tube. For example, purple or purple top tubes contain EDTA; those with a light blue cap contain citrate, etc.

What color tubes are used for samples?

The international color code for the different presentations of the tubes for sampling is as follows:

  • Red cap: Without anticoagulant (dry tube)
  • Lilac cap: with EDTA (hematic squares)
  • Blue cap: with sodium citrate(coagulation)
  • Green or white cap: with heparin.
  • Gray cap: with oxalate.

What are the tubes used in the clinical laboratory?

In clinical laboratory testing of venous blood samples, blood collection tubes are divided into three main groups: serum tubes, plasma tubes, and whole blood tubes. The choice of tube depends on what you want to achieve with the blood sample.

What do laboratory tubes contain?

These tubes contain EDTA, a potassium salt that serves as an anticoagulant, which is scattered on the walls of the tube. This is used to store the blood sample (whole) when hematological tests (blood cell count) are to be carried out or smears to be seen under a microscope.

How is citrate obtained?

Sodium citrate is obtained by the chemical reaction of critical acid and sodium bicarbonate.

What is the best time to take magnesium and zinc?

Magnesium supplements should be taken at certain times of the day to promote its absorption. Usually first thing in the morning and at night before bed. Magnesium is an essential mineral for carrying out many metabolic processes.

What side effects does magnesium citrate have?

Other side effects that may occur are vomiting and nausea, although these are rare. Hypermagnesemia (excess magnesium) is a rare but serious electrolyte disorder and needs to be treated immediately.

How to prepare tubes with sodium citrate?

Since sodium citrate has very little toxicity, it is also used for blood storage. The mixing ratio is 1 part sodium citrate to 9 parts blood. Therefore, it is essential that the bottle is filled to the line to ensure that the tests are interpreted accurately.

What does sodium citrate inhibit?

It is an endogenous or exogenous substance that infers or inhibits blood coagulation, creating an antithrombotic state.

What is Cpda anticoagulant?

Citrate Phosphate Dextrose Adenine (CPDA-1) It is an anticoagulant solution that prevents coagulation and preserves the blood. Adenine helps maintain high levels of ATP in red blood cells. Additionally the PH is adjusted. It allows to preserve the blood for 35 days.